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THE SAN ANTONIO NATHAN SHOCK CENTER FOR
EXCELLENCE IN THE BASIC BIOLOGY OF AGING
Oxidative Stress Core
Levels of F2-isoprostanes and DNA
oxidation in liver from young and old rats. F2-isoprostanes
and oxo8dG levels were measured in liver tissue from young (6 mo) and old
(24 mo) ad libitum fed (AL)
or caloric restricted rats (CR). Both markers of oxidative damage were
found to increase significantly with age, and caloric restriction prevented
this increase.
Holly Van Remmen, PhD
Core Leader
Asish Chaudhuri, PhD
Co-Leader
Tel: 210-562-6141
E-mail: vanremmen@uthscsa.edu
The Oxidative Stress Core provides investigators with reliable and reproducible measures of oxidative damage to cellular components (DNA, lipid, and protein). The Core offers important services to investigators as we employ the latest and most sensitive techniques using the most sophisticated technologies and equipment not readily available in the laboratories of individual investigators.
Core services include:
- Measurement of F2-isoprostanes, a marker of lipid peroxidation in plasma, tissues, or urine by GC-MS
(Roberts and Morrow, Free Radical Biology & Medicine: 2000, Vol. 28, No. 4, 505-513; Ward et al., Journal of Gerontology: 2005, Vol. 60A, No. 7, 847-851)
- Measurement of oxidative damage to DNA using HPLC-based analysis of oxo-8dG (Hamilton et al., Nucleic Acids Research: 2001, Vol. 29, No. 10, 2117-2126)
- Measurement of oxidative damage to proteins using a novel fluorometric-based assay to detect protein carbonyls (Chaudhuri et al., Mechanisms of Ageing and Development: 2006, 127, 849-861)
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