Victoria Centonze Frohlich, Ph.D., Associate Professor/Research
Dartmouth College
1985

(210) 567-3151
Frohlich@uthscsa.edu

Associate Director, Core Optical Imaging Facility
Director of Institutional Research Cores

Dr. Frohlich's training is as a Cell Biologist with her greatest interest in the areas of cell motility and early development. Throughout her career she has applied a variety of microscopy techniques to her research. Her experience with light microscopy includes phase-contrast and Nomarski-DIC transmitted light imaging techniques, and wide-field, confocal and multiphoton fluorescence imaging techniques. Other light microscopy methods with which she is familiar are FRAP (Fluorescence Recovery After Photobleaching), FRET (Fluorescence Resonance Enery Transfer), FLIM (Fluorescence Lifetime Imaging Microscopy), photolysis, and optical trapping. Dr. Frohlich also has experience with transmission and scanning electron microscopy.

As the Associate Director of the Optical Imaging Facility, an institutional core research facility for UTHSCSA, Dr. Frohlich is responsible for consulting with clients of the facility on experimental design and specimen preparation, training clients on the theory and usage of the optical systems, maintenance and upgrading existing equipment and introduction of new technologies to the facility..

Publications
Firulli, B.A., D. Krawchuk, V.E. Centonze, N. Vargesson, D. M. Virshup, S.J. Conway, P. Cserjesi, E. Lauger, A.B. Firulli. (2005) Altered Twist1 and Hand2 dimerization is associated with Saethre-Chotzen syndrome and limb abnormalities. Nature Genetics 37: 373-381.

Herman, B., R.V. Krishnan, V.E. Centonze. (2004) Microscopic Analysis of Fluorescence Resonance Energy Transfer. Methods in Molecular Biology, Vol.261 : Protein-protein interactions; Methods and Protocols, (Ed) H. Fu (Humana Press Inc. NJ), pp 351-370.

Centonze, V.E., B.A. Firulli, A.B. Firulli. (2004) Fluorescence Resonance Energy Transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins. Biol. Proced. Online 6: 78-82.

Centonze V. E., M. Sun, A. Masuda, H. Gerritsen, B. Herman. (2003) FRET Imaging Microscopy. Methods in Enzymology 360: 542-60.

Herman, B, G. Gordon, N. Mahajan, and V.E. Centonze. (2001) "Measurement of Fluorescence Resonance Energy Transfer in the Optical Microscope" In Methods in Cellular Imaging (ed. A Periasamy) Oxford University Press, London.

Takahashi, A., Y. Zhang, V.E. Centonze and B. Herman. (2001) Measurement of mitochondrial pH in situ. Biotechniques 30: 804-815.

Centonze, V.E. (2002) Introduction to Multiphoton Excitation Imaging for the Biological Sciences. In: Confocal Microscopy. (ed. Brian Matsumoto) Academic Press. Methods in Cell Biology 70:129-148

Centonze, V.E. and J.G. White. (1998) Multiphoton excitation provides optical sections from deeper within scattering specimens than confocal imaging. Biophys. J. 75:2015-2024.