Cellular and Structural Biology | Research Cores
Department of Cellular & Structural Biology

Optical Imaging Facility Instrumentation

 

Confocal / Multiphoton Microscopy
System 1
Olympus FV-1000
Laser Scanning Confocal Microscopy
Olympus IX-81 Microscope

Objectives:
Lasers:
  • 10X 0.40 NA (UPlanSApo, DIC)
  • 20X 0.75 NA (UPlanApo, DIC)
  • 40X 0.60 NA (LUCPlanFLN, DIC)
  • 40X 1.30 NA (UPlanFLN, Oil, DIC)
  • 40X 1.00 NA (UPlanApoN, Oil, DIC)
  • 60X 1.42 NA (PlanApoN, Oil, DIC)
  • 60X 1.10 NA (LUMFI, Water) - Available upon request
  • Blue Diode (405nm)
  • Argon (458nm, 488nm, 515nm)
  • Green HeNe (543nm)
  • Red Diode (635nm)
  • Chameleon Multiphoton tunable laser, 715nm - 900nm

    • Spectral scanning
    3 Channel Detection for Fluorescence
    Brightfield Detection of DIC images
    Z-stepper motor
    Fluoview and Tiempo Ratio Imaging Software
    LSM Technology Objective Inverter

     

    System 2
    Zeiss 510 NLO
    (Confocal and Multiphoton)
    Zeiss Axiovert 200 Microscope

    Objectives:
    Lasers:
  • 10X 0.30 NA (Plan-NEOFLUAR)
  • 40X 1.30 NA (Plan-NEOFLUAR, Oil, DIC)
  • 63X 1.40 NA (Plan-ApoCHROMAT, Oil, DIC)
  • 25X - Available upon request
  • 40X Water Immersion - Available upon request
  • 63X Water Immersion - Available upon request
  • 100X Oil - Available upon request
  • Argon (458/488/514 nm; 25 mW)
  • Green HeNe (543nm; 1 mW)
  • Red HeNe (633nm; 5 mW)
  • Coherent MIRA/5W VERDI fs TiS for multiphoton excitation imaging

    • Non-descanned Detection Single Channel for MP detection
    Z-stepper Motor
    Physiology Software
    LSM Flexible Scan Package
    3D Image Analysis
    Becker-Hickl SPC-730 FLIM detector (NDD) and analysis software
    LSM Technology objective inverter

     

    System 3
    Multiphoton Imaging Instrumentation Testbed System
    Olympus IX-70 Microscope

    Objectives:
  • 10X 0.3 NA UpanFl
  • 40X 1.3 NA UplanApo
  • 60X 1.2 NA Water Immersion
  • 100X 1.35 NA UplanApo

    • Customized Olympus Fluoview X Scanhead and software
    2 Channel Detection with Non-descanned external PMTs
    Hamamatsu Streak Camera FLIM imaging system and software
    LIMO (Nikon) FLIM detector and software
    Z-stepper motor
    Coherent MIRA/10W VERDI fs Ti:S for multiphoton excitation imaging
    Coherent 9200 Pulse-picker
    APE Pulse Check Autocorrelator
    Reese Monitor

     

    Widefield Microscopy
    System 1
    Olympus IX-70 Microscope

    Objectives:
  • 10X 0.3 NA (UplanFl, Ph)
  • 20X 0.5 NA (UplanFl, Ph)
  • 40X 0.75 NA (UplanFl, Ph)
  • 100X 1.35 NA (UPlanApo, DIC)

    • Hamamatsu Orca II 14 bit camera
    Sutter Lambda 10-2 Filter Wheels in the Excitation and Emission Pathways
    Immunofluorescence Filters: DAPI/FITC/RITC/GFP/BFP/CFP/YFP/DsRED

     

    System 2
    Nikon Eclipse 2000

    Objectives:
  • 10X 0.45 NA (PlanApo, DIC)
  • 20X 0.75 NA (PlanApo, DIC)
  • 40X 1.30 NA (S Fluor, Oil)
  • 60X (PlanApo TIRF, Oil, DIC)

    • PARISS Spectral Imaging System
    Photometric CoolSnap HQ2 Camera
    NIS Elements Software

     

    Fluorescence Stereo Microscopy
    System 1
    Leica MZ16 FA Fluorescence Stereomicroscope
    16:1 zoom,
    Basic magnification
    Range 7.1x to 11.5x
    FluoCombi attachment

    Objectives:
  • 0.5X
  • 1.0X
  • 4X

    • Fluorescence Filters: CFP, GFP, YFP, DsRed
    Transmitted, Fluorescence, Goose-neck light sources
    Image-ProPlus Analytical Imaging Software
    3D-Topography Analysis Software
    In-Focus Automation Software
    Hamamatsu Orca-AG camera
    CRI LCD Filter

     

    Whole Animal Imaging (Bioluminescence, Fluorescence)
    System 1
    IVIS Spectrum
    Fluorescence Filters
    Excitation - 10 (415 nm -760 nm (30 nm bandwidth each))
    Emission - 18 (490 nm -850 nm (20 nm bandwidth each))
    Quantum Efficiency
    >85% 500 - 700 nm
    >30% 400 - 900nm
    Lens
    f/1 - f/8
    Field of View (FOV)
    Minimum FOV - 3.9 x 3.9 cm
    Maximum FOV - 23 x 23 cm (up to 5 mice)
    Minimum Detectable Radiance
    70 photons/s/cm2/sr
    Stage Temperature
    20 - 40°C
    Transillumination Stage
    Isoflurane Gas Anesthesia
    external chamber, internal nose cones
    Dimensions
    Imaging Chamber Interior Size: 43Wx50Dx60H cm (17 x 20 x 24 in)
    Plate reader insert

     

    Ancillary Equipment
    Isofluorane anesthetizing machine
    Mini-vent ventilator
    Mouse Ox dosimeter
    CO2 Incubator
    Bioptechs stage incubator chambers
    Imaging system diagnostic equipment
    LSM Technologies Objective Inverters
    Heater stages
    Stage Incubator
    Sinks
    Small workbench

     

    Publication Acknowledgements:

    The facility should be listed in the "Acknowledgements" section of any publication using data generated in the OIF as follows:
      "Images were generated in the Core Optical Imaging Facility which is supported by UTHSCSA, NIH-NCI P30 CA54174 (CTRC at UTHSCSA), NIH-NIA P30 AG013319 (Nathan Shock Center) and NIH-NIA P01AG19316."