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Confocal / Multiphoton Microscopy

System 1 - Olympus FV-500 Laser Scanning Confocal Microscopy
Olympus FV-500 Group Calendar
Olympus IX-81 Microscope

Objectives:

• 10X 0.40 NA (UPlanSApo, DIC)
• 20X 0.75 NA (UPlanApo, DIC)
• 40X 0.60 NA (LUCPlanFLN, DIC)
• 40X 1.30 NA (UPlanFLN, Oil, DIC)
• 40X 1.00 NA (UPlanApoN, Oil, DIC)
• 60X 1.42 NA (PlanApoN, Oil, DIC)
• 60X 1.10 NA (LUMFI, Water) – Available upon request

Lasers:

• Blue Diode (405nm)
• Argon (458nm, 488nm, 515nm)
• Green HeNe (543nm)
• Red HeNe (633nm)

3 Channel Detection for Fluorescence
Brightfield Detection of DIC images
Z-stepper motor
Fluoview and Tiempo Ratio Imaging Software

. . . .

System 2 - Zeiss 510 NLO (Confocal and Multiphoton)
Zeiss 510 Group Calendar
Zeiss Axiovert 200 Microscope

Objectives:

• 10X 0.30 NA (Plan-NEOFLUAR)
• 40X 1.30 NA (Plan-NEOFLUAR, Oil, DIC)
• 63X 1.40 NA (Plan-ApoCHROMAT, Oil, DIC)
• 25X – Available upon request
• 40X Water Immersion - Available upon request
• 63X Water Immersion - Available upon request
• 100X Oil - Available upon request

Lasers:

• Argon (458/488/514 nm; 25 mW)
• Green HeNe (543nm; 1 mW)
• Red HeNe (633nm; 5 mW)
• Coherent MIRA/5W VERDI fs TiS for multiphoton excitation imaging

Non-descanned Detection Single Channel for MP detection
Z-stepper Motor
Physiology Software
LSM Flexible Scan Package
3D Image Analysis
Becker-Hickl SPC-730 FLIM detector (NDD) and analysis software
LSM Technology objective inverter

. . . .

System 3 - Multiphoton Imaging Instrumentation Testbed System
Olympus IX-70 Microscope

Objectives:

• 10X 0.3 NA UpanFl
• 40X 1.3 NA UplanApo
• 60X 1.2 NA Water Immersion
• 100X 1.35 NA UplanApo

Customized Olympus Fluoview X Scanhead and software
2 Channel Detection with Non-descanned external PMTs
Hamamatsu Streak Camera FLIM imaging system and software
LIMO (Nikon) FLIM detector and software
Z-stepper motor
Coherent MIRA/10W VERDI fs Ti:S for multiphoton excitation imaging
Coherent 9200 Pulse-picker
APE Pulse Check Autocorrelator
Reese Monitor

Widefield Microscopy

System 1 - Olympus IX-70 Microscope
OlympusWF Group Calendar

Objectives:

• 10X 0.3 NA (UplanFl, Ph)
• 20X 0.5 NA (UplanFl, Ph)
• 40X 0.75 NA (UplanFl, Ph)
• 100X 1.35 NA (UPlanApo, DIC)

Hamamatsu Orca II 14 bit camera
Sutter Lambda 10-2 Filter Wheels in the Excitation and Emission Pathways
Immunofluorescence Filters: DAPI/FITC/RITC/GFP/BFP/CFP/YFP/DsRED

. . . .

System 2 - Nikon Eclipse 2000

Objectives:

• 10X 0.45 NA (PlanApo, DIC)
• 20X 0.75 NA (PlanApo, DIC)
• 40X 1.30 NA (S Fluor, Oil)
• 60X (PlanApo TIRF, Oil, DIC)

PARISS Spectral Imaging System

Fluorescence Stereo Microscopy

Leica MZ16 FA Fluorescence Stereomicroscope
Leica MZ16 Group Calendar
16:1 zoom, Basic magnification range 7.1x to 11.5x
FluoCombi attachment

Objectives:

• 0.5X
• 1.0X
• 4X

Fluorescence Filters: CFP, GFP, YFP, DsRed
Transmitted, Fluorescence, Goose-neck light sources
Image-ProPlus Analytical Imaging Software
3D-Topography Analysis Software
In-Focus Automation Software
Hamamatsu Orca-AG camera
CRI LCD Filter

Ancillary equipment

Heater stages
Incubator
Sinks
Small workbench

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