Hanna E. Abboud, M.D.
Professor, Department of Medicine
Chief, Division of Nephrology
| Location: | DTL, Room 5.080 |
| Phone: | (210) 567-4700 |
| Fax: | (210) 567-4712 |
| E-mail: | abboud@uthscsa.edu |
Research Interests:
Research in this laboratory focuses on the pathogenic mechanisms
involved in development and progression of renal disease. The
role of growth factors and chemokines in mediating these processes
with major emphasis on platelet-derived growth factor is being
explored. Studies in cultured kidney cell lines aim at identifying
membrane, cytoplasmic and nuclear signals involved in mediating
biologic effects of PDGF. Studies on molecular mechanisms of PDGF
gene regulation
aim to identify transcription factors and DNA elements involved
in constitutive and cytokine stimulated expression of PDGF and
PDGF beta receptors. The in vivo studies look at the expression
of signalling proteins and transcription factors during kidney
embryonic development and in animal models of renal disease.
Unique Technical and Clinical Research Capabilities/Instrumentation:
Analysis of RNA by Northern blotting solution hybridization
and in situ hybridization. Establishment of cultured cell lines
from kidney tissue. Cloning, transfection, gel retardation, DNAase
foot printing, DNA library screening. Immunoprecipitation, Western
blotting, ELISAs, RIAs.
Publications:
Ghosh Choudhury G, Ghosh Choudhury N, Abboud HE: Association
and direct activation of STATa by platelet-derived growth factor
receptor. J Clin Invest 101:2751-2760, 1998.
Grandaliano G, Ghosh Choudhury G., Poptic E, Woodruff K, Barnes
JL, Abboud HE: Thrombin regulates PDGF expression in bovine glomerular
endothelial cells. J. Am. Soc. Neph. 9:583-589, 1998.
Simon M, Cleary T.G., Herhandez J.D., Abboud H.E. Shiga toxin elicits diverse biologic responses in mesangial cells. Kidney International 54:117-1127, 1998.
Valente AJ, Xie J-F, Abramova MA, Wenzel UO, Abboud HE, Graves DT. A Complex Element Regulate Interferon-gamma-Stimulated Monocyte Chemoattractant Protein-1 Gene Transcription. J. Immunology 161:3719-3728, 1998.
Park I-S, Kiyomoto H, Alvarez F, Xu Y-C, Abboud HE, Abboud SL. Preferential Expression of Insulin-Like Growth Factor Binding Proteins-1, -3, and -5 During Early Diabetic Renal Hypertrophy in Rats. Am. J. Kid. Dis. 32:1000-1010, 1998.
Simon M., Maresh J.G., Harris S.E., Hernandez J.D., Arar M., Olson M.S., Abboud H.E. Expression of Bone Morphogenetic Protein-7 (BMP-7) mRNA in the Normal and Ischemic Adult Rat Kidney. Am. J. Physio. 276:F382-F389, 1999.
Ghosh Choudhury G, Kim Y-S, Simon M, Ghosh Choudhury N, Wozney J, Harris S, Abboud HE. Bone morphogenetic protein 2 inhibits PDGF-induced c-fos gene transcription and DNA syntheis in mesangial cells: Involvement of mitogen activated protein kinase. J. Biol. Chem. 274:10897-10902, 1999.
Mendez R, Abboud HE, Burdick J, Copley B, Freeman R, Batiuk T, Cooney GF Reduced Intrapatient Variability of Cyclosporine Pharmacokinetics in Renal Transplant Recipients Converted from Oral Sandimmuneâ to Neoralâ. Clin Ther 21:260-71, 1999.
Bardgette J, Abboud HE, Ghosh Choudhury G. Activation of STAT1a by Phosphatase Inhibitor Vanadate in Glomerular Mesangial Cells: Involvement of Tyrosine and Serine Phosphorylation. J Receptor and Signal Transduction 19:865-84, 1999.
Yang CW, Faulkner GR, Jin DC, Abboud HE, Bagby GC, Bennett WM, Andoh TF. Activation of Interleukin-1b Converting Enzyme and CPP32 Protease in Chronic Cyclosporine Nephrotoxicity. Kidney Intl. 1999.
Ghosh Choudhury G, Jin DC, Kim YSs, Celeste A, Ghosh-Choudhury N, Abboud HE. Bone morphogenetic protein-2 inhibits MAPK-dependent Elk-1 transactivation and DNA synthesis induced by EGF in mesangial cells. Biochem Biophys Res Commun May 10;258(2):490-6, 1999.
Goutam Ghosh Choudhury, Giusippe Grandaliano, Dong-Chan Jin, Michael S. Katz, and Abboud HE: Activation of PLC and PI 3 kinase by PDGF receptor is a not sufficient for mitogenesis and migration in mesangial cells; Kidney International, Vol. 57, pp. 908-917, 2000.
Arar Mazen, Y. Xu, Elshihabi Ihsan, Ghosh Chodhury Goutam,
Barnes Jeffrey L., and Abboud HE. Platelet-derived Growth Factor
Receptor b Regulates Migration and DNA Synthesis in Metanephric
Mesenchymal Cells; J Biol Chem, Vol. 275, pp. 9527-9533, 2000.
Key Words:
growth factors, kidney, signal transduction, cytokines, kidney
development