David D. Dean, Ph.D
Biochemistry - Cell Biology
I received my B.S. degree with Honors in Biology from Randolph-Macon College, Ashland, Virginia. Upon graduation, I entered graduate school at the University of North Carolina at Chapel Hill where my doctoral dissertation research involved characterizing the collagenolytic proteases elaborated by a fungal parasite (Lagenidium giganteum) of mosquito larvae. I then migrated to Miami for a postdoctoral fellowship in Dr. J. Frederick Woessner’s laboratory in the Department of Biochemistry at the University of Miami School of Medicine. During my training in Miami, I received an Arthritis Foundation postdoctoral fellowship to develop methods for the extraction and quantitation of matrix metalloproteinases from normal and osteoarthritic cartilage. Upon completion of the fellowship I remained at the University of Miami and joined Dr. David Howell’s laboratory where we continued examining the regulation of matrix metalloproteinases in osteoarthritis and their involvement in cartilage calcification in growth plate. I moved to San Antonio in 1992 to continue my interest in growth plate cartilage with Drs. Barbara Boyan and Zvi Schwartz. Since 2002, I have expanded my interests into the field of biomedical engineering and cell response to materials.
My current research activities focus on three main areas. The first area of interest involves examining how osteoblasts interact with titanium implant surfaces and the role of arachidonic acid metabolites (prostaglandin E2, arachidonic acid, and nonsteroidal antiinflammatory drugs) in regulating osteoblast response. In addition, the laboratory is developing methods for measuring changes in gene expression during the first 3-6 hours of culture on an implant surface so that it will be possible to examine some of the very earliest cell responses to titanium or other biomaterials. A second area focuses on examining osteoblast response to particulate wear debris (UHMWPE and Ti), including changes in gene expression, and methods for characterizing and isolating wear debris. The third area of research is a joint effort with Dr. Mauli Agrawal’s laboratory and involves developing methods for assessing factors, such as material chemistry or architecture, that regulate cell attachment, proliferation, and differentiation on tissue engineering scaffolds and then incorporating this knowledge into a framework for the rational design of scaffolds.
In addition these various research endeavors, I am the Chairman of both the Orthopaedic Resident Research Committee and the Committee on Graduate Studies for the Biomedical Engineering Program (http://bme.uthscsa.edu). I also serve on the Health Science Center’s Institutional Chemical Safety committee and on the Editorial Advisory Board of Osteoarthritis and Cartilage.
Dean DD, Schwartz Z, Liu Y, et al.. The Effect of Ultrahigh Molecular Weight Polyethylene Wear Debris on MG63 Osteosarcoma Cells In Vitro. J Bone and Joint Surgery (Am Vol) 81:452-461, 1999.
Dean DD, Lohmann CH, Sylvia VL, et al.: Effect of Polymer Molecular Weight and Addition of Calcium Stearate on Response of MG63 Osteoblast-like Cells to UHMWPE Particles. J Orthopaedic Research 19:179-186, 2001.
Maeda S, Dean DD, Gay I, et al.: Activation of Latent Transforming Growth Factor-ß 1 by Stromelysin-1 in Extracts of Growth Plate Chondrocyte-derived Matrix Vesicles. J Bone and Mineral Research 16:1281-1290, 2001.
Lohmann CH, Dean DD, Bonewald LF, et al.: Production of Nitric Oxide and Prostaglandin E2 by Osteogenic Cells in Response to Ultra-high Molecular Weight Polyethylene Particles is Dependent on Cell Maturation State. J Bone and Joint Surgery (Am Vol) 84:411-419, 2002.
Kim HJ, Kim SH, Kim MS, et al.. Varying Ti-6Al-4V Surface Roughness Induces Different Early Morphologic and Molecular Responses in MG63 Osteoblast-like Cells. Journal of Biomedical Materials Research, 74A:366-373, 2005.