Eloise Dray, Ph.D.
Biochemistry, Breast Cancer, Cell Biology
DNA Damage Repair; Chromosomes Structure
Our DNA is constantly exposed to DNA damaging agents of internal and external origin. Several mechanisms exist in mammalian cells to repair various DNA damages that would otherwise accumulate, destabilize the genome, and eventually lead to cancer.
Among all damages that can occur, Double Stranded-DNA breaks (DSBs) are the most deleterious for humans and are efficiently repaired by Homologous Recombination (HR) or Non-Homologous End Joining (NHEJ). Importantly, inherited mutations in HR genes are often responsible for familial breast or ovarian cancers. These mutations predispose to early onsets of cancer and particularly aggressive subtypes of tumors.
Related Diseases: Familial breast and ovarian cancer, hereditary pancreatic cancer, congenital anomalies.
Techniques: Molecular Biology (PCR, cloning, sequencing, northern blots, mutagenesis) , Biochemistry (Protein expression and purification, gel filtration, chromatography, western blots, broad range of biochemical assays) , Cytology (immuno-localization, recombination assays, confocal and epifluorescence microscopies), Tissue culture and response to genotoxics and irradiations.