Mark  S. Shapiro, Ph.D.

Physiology and modulation of ion channels in brain and peripheral nerve, and role in disease

Neuroscience, Epilepsy, Neurotrauma, Pain, Stroke, Cognitive Function, Drug Addiction

My research program spans the physiology and modulation of voltage-gated K+ and Ca2+ channels, and  TRP cation and Ano1/2 Cl- channels in neurons and non-excitable cells.  We have focused on signal transduction pathways of G protein-coupled receptors, using electrophysiology of native neurons and heterologous systems, biochemistry, confocal and advanced microscopy, molecular biology and live single-cell and whole-animal imaging. Our lab is both basic science and translational, spanning molecular biophysics to behavior.

Related diseases: epilepsy, drug addiction, pain, traumatic brain injury, stroke, drug addiction, cognitive dysfunction, cardiovascular control, psychiatric disease, brain development, hearing loss/tinnitus, and others.

Techniques: Patch clamp electrophysiology of native neurons brain slice and heterologous systems, advanced microscopy of single living cells using confocal and single-molecule TIRF techniques, super-resolution microscopy, immunochemistry, biophysical chemical analysis, structural biology including X-ray crystallography and NMR spectroscopy, immunochemistry including immunoblots and immunohistochemistry, molecular biology, transgenic animals, model animals in disease states, animal behavior, transcriptional analysis using PCR and multiplex.

• Professional Background

  Education

  • 1991 - PhD - Physiology - Rush University
  • 1984 - BA - Physics - University of Chicago
  • Postdoctoral Fellowship - Physiology/Biophysics - University of Washington

  Appointments

  • 9/2010 - Professor - University of Texas Health Science Center, Physiology, San Antonio

• Instruction & Training

  • - Present, Membership on Supervising Committee, UTHSCSA
  • - Present, Membership on Supervising Committee, UTHSCSA
  • 2/2015 - Present, Dental Physiology, The University of Texas Health Science Center
  • 1/2014 - Present, Medical Physiology, The University of Texas Health Science Center
  • 1/2010 - Present, IMGP Core Course, The University of Texas Health Science Center
  • 1/2010 - Present, Excitable Membranes, The University of Texas Health Science Center
  • 1/2008 - Present, Biochemistry, The University of Texas Health Science Center
  • 1/2007 - Present, Selected Topics, The University of Texas Health Science Center
  • 1/2007 - Present, Signaling in Molecular Physiology, The University of Texas Health Science Center
  • 1/2007 - Present, Ion Channel Research, The University of Texas Health Science Center
  • 1/2006 - Present, PHYL 5045 Mammalian Physiology, The University of Texas Health Science Center
  • 1/2005 - Present, Pre-Doctoral Student Supervision, UTHSCSA
  • 1/2005 - Present, Physiology, The University of Texas Health Science Center
  • 1/2004 - Present, Ion Channel Research, The University of Texas Health Science Center
  • 1/2003 - Present, Research in Neuroscience, The University of Texas Health Science Center
  • 7/2002 - Present, Undergraduate Student Supervision
  • 1/2002 - Present, Cell and Molecular Physiology, The University of Texas Health Science Center
  • 1/2001 - Present, Post-Doctoral Student Supervision, University of Texas Health Science Center
  • 1/2001 - Present, Cell Structure and Function, The University of Texas Health Science Center

• Research & Grants

  Research profile

  Grants

  Private

  Funding Agency British Heart Foundation Title Defining the role of phosphatidyl 4, 5 bisphosphate on arterial calcium-activated chloride channels Status Active Period 1/2015 - 10/2018 Role Co-Investigator Grant Detail

• Publications

  Book Chapter

  Gamper, N., Shapiro MS. KCNQ Channels, Jie Zheng and Matt Trudeau, eds. In: Jie Zheng and Matt Trudeau, eds. Handbook of Ion Channels, Chapter 20. Boca Rotan, FL: CRC Press, Boca Rotan, Fl; 2015.

  Journal Article

  Zhang, J., Choveau F. and M.S. Shapiro. Clustering and functional coupling of diverse ion channels and signaling proteins revealed by super-resolution STORM microscopy in neurons Neuron (in revision) 2016 Jan;. Choveau, F., Hernandez, C., C., Bierbower, S.M. and M. S. Shapiro. Phosphatidylinositol 4,5-bisphosphate (PIP2) regulates KCNQ3 K channels through distinct sites of action Journal of General Physiology (In revision) 2016 Jan;. Choveau, F., and M. S. Shapiro. The helix C-D linker determines KCNQ3 current amplitudes by controlling channel trafficking PLoS One 2016 Jan;10(12). Choveau FS, Zhang J, Bierbower SM, Sharma R, Shapiro MS. The Role of the Carboxyl Terminus Helix C-D Linker in Regulating KCNQ3 K+ Current Amplitudes by Controlling Channel Trafficking PLoS One 2015 Dec;10(12):0145367-0145367. Zhang, J, Shapiro MS. Novel roles of AKAP79/150 in orchestrating multi-protein signaling complexes in brain and peripheral nerve Journal of Physiology 2015 Feb;. Beirbower, S.M., Shapiro MS. Foster resonance energy transfer-based imaging at the cell surface of live cells 2013 Jan;. Chaudhury, S., Manjot Bal, M., Shapiro MS, Jeske NA. AKAP150-mediated TRPV1 sensitization ins disrupted by Ca 2+/calmodulin Mulecular Pain 2011 Jan;7(34).