James Stockand, Ph.D.

Research in the Stockand laboratory focuses on ion channels and ion transport. We are particularly interested in the proteins involved in controlling ion movement into neurons and epithelial cells. The prior is important to excitability and how central neurons signal to each other and how peripheral neurons allow us to sense our environment.  Ion channels in the kidney play a central role in controlling blood pressure as they set circulatory volume and the water and electrolyte content of plasma. We focus on understanding biologically important mechanisms that affect physiology. The lab has a long publication history on the mechanisms of regulation, structure-function relations and physiologic function of Deg/ENaC ion channels, in which we have employed electrophysiology, live-cell optical/fluorescence imaging, biochemical and molecular biological approaches, as well as contemporary molecular genetics and assessment of renal function, blood pressure and behavior in live animals.

• Professional Background

  Education

  • 2000 - Postdoctoral Fellowship - Physiology/Biophysics - Emory University School of Medicine
  • 1996 - PhD - Physiology/Integrative Biology - The University of Texas Health Science Center
  • 1991 - BS - Biochemistry and Genetics - Texas A&M University

  Appointments

  • 9/2009 - Professor - UTHSCSA, Physiology, San Antonio

• Instruction & Training

  • 1/2017 - Present, Ph.D. Dissertations Directed, UTHSCSA
  • 1/2015 - Present, Physiology, The University of Texas Health Science Center
  • 1/2013 - Present, Renal/Acid Base Physiol, The University of Texas Health Science Center
  • 1/2013 - Present, Physiology, The University of Texas Health Science Center
  • 4/2008 - Present, Post-Doctoral Student Supervision, UTHSCSA
  • 1/2008 - Present, Post-Doctoral Student Supervision, UTHSCSA
  • 8/2007 - Present, Ph.D. Dissertations Directed, University of Ulster, Ireland
  • 1/2007 - Present, Membership on Supervising Committee, UTHSCSA
  • 1/2007 - Present, Selected Topics, The University of Texas Health Science Center
  • 1/2006 - Present, Post-Doctoral Student Supervision, UTHSCSA
  • 1/2004 - Present, Post-Doctoral Student Supervision, UTHSCSA
  • 1/2004 - Present, Ion Channel Research, The University of Texas Health Science Center
  • 1/2001 - Present, Physiology, UTHSCSA
  • 1/2001 - Present, Physiology, The University of Texas Health Science Center
  • 1/2001 - Present, Experiments in Physiology I, The University of Texas Health Science Center

• Research & Grants

  Research in the Stockand laboratory focuses on ion channels and ion transport. We are particularly interested in the proteins involved in controlling ion movement into neurons and epithelial cells. The prior is important to excitability and how central neurons signal to each other and how peripheral neurons allow us to sense our environment.  Ion channels in the kidney play a central role in controlling blood pressure as they set circulatory volume and the water and electrolyte content of plasma.

  Research profile

  Grants

  Federal

  Funding Agency NIH/NHLBI Title Pathobiology of Occlusive Vascular Disease Status Active Period 9/2007 - 8/2022 Role Principal Investigator Grant Detail Funding Agency NIH Title Regulation of ENaC by Casein Kinase 2 Status Active Period 7/2018 - 6/2022 Role Principal Investigator Grant Detail Funding Agency NIH/NIDDK Title Regulation of renal Na handling in the collecting duct by local purinergic tone. Status Active Period 6/2010 - 5/2022 Role Principal Investigator Grant Detail Funding Agency NIH/NHLBI Title Integrating Novel Mechanisms Controlling Sodium Excretion and Blood Pressure Status Active Period 1/2017 - 1/2022 Role Co-Investigator Grant Detail Funding Agency NIH/NHLBI Title Nox1/Nox1A Regulation of Vascular Reactivity and Sodium Excretion in Health Status Active Period 1/2018 - 1/2022 Role Co-Investigator Grant Detail Funding Agency NIH/NIDDK Title Defining the contribution of ENaC to ADH-mediated water and sodium excretion Status Active Period 1/2016 - 1/2021 Role Co-Investigator Grant Detail

  Private

  Funding Agency Am. Soc. Physiol. Title Lazaro J. Mandel Young Investigator Award Status Complete Period 1/2002 - Present Role Principal Investigator Grant Detail Competitive Award Funding Agency American Heart Association Title Purinergic regulation of ENaC Status Active Period 1/2017 - 12/2018 Role Principal Investigator Grant Detail Funding Agency Cancer Prevention Research Institute of Texas Title Cellular mechanisms of chemotherapy-induced peripheral neuropathy Status Complete Period 1/2015 - 1/2018 Role Principal Investigator Grant Detail

• Publications

  Book Chapter

  Stockand JD. Degenerin/ENaC channels. In: Trudea, M. and Zheng, J. eds. Handbook of Ion Channels. Florence, KY: CRC Press; 2015.

  Editorial

  Stockand JD. 2017 Young Investigator Award of the American Physiological Society Renal Section AJP:Renal 2017 Jan;. Berman, J.M. and Stockand, J.D. Salt and water loss in the distal colon J. Physiol 2015 Jan;593.

  Journal Article

  Mironova, E., Suliman, F. and Stockand, J.D. Renal Sodium Excretion Consequent to Targeted Pharmacogenetic Activation of Gq-DREADD in Principal Cells. Submitted to Am. J. Physiol.:Renal Am. J. Physiol.:Renal 2019 Jan;. Archer, C.R., Green, R.R., Enslow, B.T., Chen, Y. and Stockand, J.D. ENaC is regulated by PIP2 through weak electrostatic interactions on multiple binding sites. In preparation for J. Biol. Chem JBC 2019 Jan;. Berman, J.M., Mironova, E. and Stockand, J.D. Regulation of the Epithelial Na Channel by Ankyrin-3 Downstream of Casein Kinase II Phosphorylation. In preparation for Am. J. Physiol.: Renal Physiol Am. J. Physiol.: Renal Physiol 2019 Jan;. Boiko, N., Pugh, J. Kreko, T. and Stockand, J.D. Ataxia Caused by Acid-Sensing Ion Channel 5 Dysfunction in Vestibulocerebellum Unipolar Brush Cells. In preparation for PLOS ONE Plos One 2019 Jan;. Ramkumar, N, Stuart, D., Mironova, E., Abraham, N., Gao, Y., Wang, S., Lakshmipathi, J., Stockand, J.D., and Kohan, D.E
  . Collecting Duct Principal, But Not Intercalated, Cell Prorenin Receptor Regulates Renal Sodium and Water Excretion Am. J. Physiol.: Renal Physiol 2018 May;315:F607-F617. Boiko, N., Medrano, G., Montano, E., Jiang, N., Williams, C.R., Madungwe, N.B., Bopassa, J.C., Kim, C.C.,
  Parrish, J.Z., Hargreaves, K.M., Stockand, J.D.1 and Benjamin A. Eaton1. 1These authors contributed
  equally
  . TrpA1 activation in peripheral sensory neurons underlies the ionic basis of pain hypersensitivity in response to vinca alkaloids Plos One 2017 Nov;12(10). Hyndman, K.A., Mironova, E.V., Giani, J.F., Dugas, C., Collins, J., McDonough, A.A., Stockand, J.D.,
  and Pollock , J.S
  . Collecting Duct Nitric Oxide Synthase 1i?? Activation Maintains Sodium Homeostasis During High Sodium Intake Through Suppression of Aldosterone and Renal Angiotesin II Pathways J. Am. Heart Assoc 2017 Oct;6. Berman, J.M., Mironova, E. and Stockand, J.D. Physiological regulation of the epithelial Na channel by Casein Kinase II Am. J. Physiol.: Renal Physiol 2017 Aug;314:F367-F372. Boiko, N., Berman, J., Mironova, E. and Stockand, J.D. Loop diuretics are K -sparing in the presence of a low-Na , high-K diet Kidney Int 2017 May;92:786-787. Mironova, E., Lynch, I.J., Berman, J., Gumz, M.L., Stockand, J.D. and Wingo, C.S. ENaC activity in the cortical collecting duct of HKi?!1 H ,K -ATPase knockout mice is uncoupled from Na intake Am. J. Physiol 2017 Apr;312:F1073-F1080. Wingo, C.S. and Stockand, J.D. Alkaline activation of ClC-K2 chloride channels switches renal cells from reabsorbing to secreting J. Gen. Physiol 2016 Sep;148:195-199. Ramkumar. N., Stuart, D., Mironova, E., Bugay, V., Wang, S., Abraham, N., Ichihara, A., Stockand, J.D., and Kohan, D.E
  . Renal tubular epithelial cell prorenin receptor regulates blood pressure and sodium transport Am. J. Physiol 2016 Apr;311:F186-F194. Boiko, N., Kucher, V., and Stockand, J.D. Pseudohypoaldosteronism type 1 and Liddle?s syndrome mutationsthat affect the single-channel properties of the epithelial Na channel Physiol. Reports 2015 Sep;3. Akitake, B., Ren, Q., Boiko, N., Ni, J., Sokabe, T., Stockand, J.D., Eaton, B.A. and Montell, C. Coordination and Fine Motor Control Depends on Drosophila TRPy Nature Comm 2015 Jul;6. Mironova, E., Boiko, N., Bugaj, V., Kucher, V. and Stockand, J.D. Regulation of Na excretion and arterial blood pressure by purinergic signaling intrinsic to the distal nephron: consequences and mechanisms Acta Physiologica 2015 Jun;213:213-221. Mironova, E., Chen, Y., Pao, A.C., Roos, K.P., Kohan, D.E., Bugaj, V. and Stockand, J.D. Activation of ENaC by AVP contributes to urinary concentrating mechanism and dilution of plasma Am. J. Physiol 2015 May;308:F237-F243. Hyndman, K., Bugaj, V., Mironova, E., Stockand, J.D. and Pollock, J. NOS1-dependent negative feedback regulation of the epithelial sodium channel in the collecting duct Am. J. Physiol 2015 Apr;308:F244-F254.