Extracellular vesicles (EVs) have great potential as a cell-free therapy in wound healing applications. Because EV populations are not equivalent, rigorous characterization is needed before clinical use. Although there has been much focus on their RNA composition and regenerative capabilities, relatively less is known regarding the effects of cell type and culture condition on EV performance. As such, I seek to characterize the functionalities of EVs isolated under various conditions in hopes of down-selecting for specific sub-groups of EVs best suited for use in different forms of trauma.
Chance TC, Herzig MC, Christy BA, Delavan C, Rathbone CR, Cap AP, Bynum JA. Human mesenchymal stromal cell source and culture conditions influence extracellular vesicle aniogenic and metabolic effects on human endothelial cells. J Trauma Acute Care Surg. 2020. (In press).
Chance TC, Rathbone CR, Kamucheka RM, Peltier GC, Cap AP, Bynum JA. The effects of cell type and culture condition on the procoagulant activity of human mesenchymal stromal cell-derived extracellular vesicles. J Trauma Acute Care Surg. 2019;87(1S Suppl 1):S74-S82.
M.S.E., Chemical and Biomolecular Engineering, Johns Hopkins University, 2017
B.S., Chemical and Biomolecular Engineering, Johns Hopkins University, 2016
M.S., Chemical and Biomolecular Engineering, The Johns Hopkins University, 2017
B.S., Chemical and Biomolecular Engineering, The John Hopkinds University, 2016