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Brij Singh | UT Health San Antonio

Contact

210-567-3798

singhbb@uthscsa.edu

Departments & Divisions

Department of Periodontics

Institutes and Centers

Currently seeking Ph.D. students.

Brij B Singh, Ph.D.

Associate Dean for Research

  • Professional Background

    Education

    • 2002 - Postdoctoral Fellowship - Gene Therapy & Therapeutics Branch - National Institutes of Health
    • 1999 - Postdoctoral Fellowship - Pharmacology & Therapeutics - Medical College of Wisconsin
    • 1998 - Postdoctoral Fellowship - Molecular Biochemistry Section - University of Bonn
    • 1997 - PhD - Biochemistry (Awarded DAAD fellowship (sandwich model) to carry out PhD research in Germany.) - Bhopal University India & the University of Bonn Germany
    • 1993 - MS - Biotechnology - Aligarh University (AMU)
    • 1991 - BS - Biology with Minor in Chemistry - Aligarh University (AMU)
    • Biology with Minor in Chemistry - Aligarh University (AMU)

    Appointments

    • 11/2017 - Associate Dean for Research - UT Health San AntonioSan Antonio
    • 11/2017 - Professor with Tenure - UT Health San Antonio, Periodontics, San Antonio

  • Instruction & Training

    • Membership on Supervising Committee, University of North Dakota
    • - Present, BIMD 503

  • Research & Grants

    Our research is directed towards, identification and regulation of the molecular mechanisms involved in Ca2+ signaling (TRPC and Orai proteins) in neuronal and non neuronal cells. Define the molecular mechanism involved in their regulation, identify the role of calcium in differential gene expression, and  in diseases such as Parkinson’s, Alzheimer, Sjogren’s, Dariers disease and Cancer.  We use cutting edge molecular, biochemical, and physiological methods for this exciting research and are also interested in developing technology for early diagnosis of these diseases.

     

    Grants

    Federal

    Current Grant Support

    Title:                  TRPC1 calcium, and exocrine function

    Source:               NIH, NIDCR                                      R01 DE017102

    Amount:             $2,791,395                                          Award period 03/01/2005-04/30/2026

    Role:                  PI: (Brij Singh) 30% effort               Co-PI   None

    The long-term goal of this project is to identify and elucidate the mechanism of the role of TRPC1 function in exocrine secretion and regulation. 20 years of continuous funding

    Title:      Glycolytic metabolites, Calcium entry and Sjogren’s syndrome

    Source:               NIH, NIDCR                                       1R01DE031716

    Amount:             $2,981,300.00                                     Award period 09/01/2022-08/31/2027

    Role:                  PI: (Brij Singh) 20% effort                Co-PI   none

    The long-term goal of this project is to elucidate the role of glycolytic metabolites in modulating calcium signaling and autoimmune diseases.

    Title:                  Epigenetic regulation in Sjögerns syndrome

    Source:               NIH, NIDCR                                      R01DE022765

    Amount:             $1,850,625                                          Award period 08/01/2017-07/31/2023

    Role:                  PI: (Brij Singh) 20% effort               Co-PI   None

    The long-term goal of this project is to elucidate the role of epigenetic regulations in immune modulation.

     

    Title:                  Lung innate immunity against bacterial infection

    Source:               NIH, NIAID                                        1R01AI109317-01A1

    Amount:             $1,770,000                                          Award period 09/01/2012-08/30/2023

    Role:                  Co-I: (Brij Singh) 5% effort              PI   Min Wu               

    The goal of this project is to determine the role of ATG-7-mediated autophagy in protection against gram-negative bacteria in bacterial clearance by alveolar macrophages.

    Title:         Titanium-induced inflammasome activation in peri-implant mucosal barrier

    Source:               NIH, NIDCR                                       1R03DE029872         

    Amount:             $425,622                                             Award period 04/01/2021 – 03/31/2023

    Role:                  Co-I: (Brij Singh) 5% effort              PI   George Kotsakis 

    The long term goal of this project is to dissect the mechanisms of implant biomaterial involvement in peri-implantitis, which has the potential to lead to the identification of pharmacological targets for therapy

     

  • Publications

    Journal Article

    • Sun, Y.,  De la Chapa, J., Zboril, EK., Nascimento Da Conceicao, V., Valdez, MC., McHardy, M., Gonzales, CB., and Singh, BB (2022). Capsazepine analog CIDD-000099 inhibits TRPC1-mediated Ca2+ entry and promote cell death in oral cancer cells.  Frontiers in Physiology (In press)

    • Claycombe-Larson, K, Singh, BB., Bundy, AN.,  Brunelle, D., Bukowski, MR and  Roemmich, JN (2022) Effects of maternal HF diet on placental growth and lipid content in TRPC1 KO mice. Journal of Nutritional Biochemistry  Elsevier Press (In press)

    • Ramachandran, K., Maity, S., Kandala,K.,  Tomar, D., Tarek,M., Allen, C., Muthukumar, AR., Sun, Y., Madaris, TR.,  Chiem, K., Truitt, R., Vishnu, N., Anderson, A., Stathopoulos, PB. Martinez, L., Yang, W., Stockand, JD., Singh, B.B., Srikantan, S., Reeves, WB.,  Madesh, M (2022). Transcriptomics Identifies SARS-CoV-2 Infection Enhances the Expression of Mitochondrial PTP Complex That Perturbs Cellular Energetics. iScience (Cell Press), Jan 21;25(1):103722. doi: 10.1016/j.isci.2021.103722.

    • Martin A., Zhou P., Singh B.B., and  Kotsakis GA (2022) Transcriptome-wide Gene Expression Analysis in Peri-implantitis Reveals Candidate Cellular Pathways JDR Clinical & Translational Research Oct;7(4):415-424.  doi: 10.1177/23800844211045297.

    • Tran, ON., Wang, H, Li, S., Malakhov, A., Yuyang,S., Abdul Azees, PA., Gonzalez, AO., Cao, B., Marinkovic, M., Singh, BB., Dean, DD., Yeh, C-Y., Chen, XD (2022). Organ-specific Extracellular Matrix Directs the Differentiation of Bone Marrow-derived Mesenchymal Stem Cells and Subsequent Formation of Salivary Gland-like Organoids In Vivo. Stem Cell Research & Therapy Jul 15;13(1):306

    • Bukowski, M., Roemmich, J.N., Singh, B.B., Claycombe-Larson, K.J (2022) Lipidomic analysis of TRPC1 Ca2+ permeable channel-knock out mouse demonstrates a vital role in placental tissue sphingolipid and triacylglycerol homeostasis under maternal high-fat diet. Frontiers in Endocrinology Mar 10;13:854269. doi: 10.3389/fendo.2022.854269

    • Ahamad N., Sun, Y., Nascimento Da Conceicao V., Ezhilan, CRD Natarajan M.,  and Singh, B.B (2021). Differential activation of Ca2+ influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration. Nature Regenerative Medicine 6, Published Oct 20.

    • Nascimento Da Conceicao, V., Sun, Y., Ramachandran, K., Chauhan, A., Raveendran, A., Venkatesan, M., DeKumar, B., Maity, S., Vishnu, N, Kotsakis, GA., Worley, P.F., Gill D.L., Mishra, B.B., Madesh, M and Singh B.B (2021). Resolving macrophage polarization through distinct Ca2+ entry channel that maintains intracellular signaling and mitochondrial bioenergetics. iScience (Cell Press) 24 (11), 103339, November 19, 2021

    • Ahamad N., Sun, Y., and Singh, B.B (2021). Increasing cytosolic Ca2+ levels restore cell proliferation and stem cell potency in aged MSCs. Stem Cell Research  56, October, doi.org/10.1016/j.scr.2021.102560

    • Sukumaran, P., Conceicao, V N., Sun, Y., Ahamad, N., Saraiva, L.R., Selvaraj, S and Singh, B.B. (2021) Calcium signaling regulates autophagy and apoptosis. Cells, 10 (8), 2125; https://doi.org/10.3390/cells10082125

    • Lee, HK.,  Donati, A., Feilerd D., Sun, Y., Ding, Y., Madesh, M., Salmon, A., Ikeno, Y., Ross, C., Connor, C.L., Ju, W., Bitzer, M., Chen, Y., Choudhury, GG., Singh, B.B., Sharma, K., and, Kasinath, BS (2021). Chloride channel accessory 1 integrates chloride channel activity and mTORC1 in aging related kidney injury. Aging Cell. Jul;20(7):e13407. doi: 10.1111/acel.13407.

    • Sharma, A., Chauhan, A.,  Tripathi, J.K., Sun, Y., Sukumran, P., Singh, B.B., Mishra, B.B and Sharma, J (2021) Helminth derived factors inhibit neutrophil extracellular trap formation and inflammation in bacterial peritonitis. Scientific Report.  Jun 16;11(1):12718.

    • Sun, Y.,  Sukumaran, P and Singh, B.B. (2021) Sigma1 receptor regulate TRPC1-mediated Ca2+ entry that inhibits cell death. Cellular and Molecular Neurobiology. Aug;41(6):1245-1255. doi: 10.1007/s10571-020-00892-5.

    • Ahamad, N and Singh, B.B (2021). Calcium channels and their role in regenerative medicine. World Journal of Stem Cells 13 (4): 260-280

    • Nemani, N, Dong, Z, Daw, C.C, Madaris, T.R, Enslow, B.T, Rubannelsonkumar, C.S, Shanmughapriya, S, Mallireddigari, V, Maity, S, SinghMalla, P, Kalimuthusamy, N, Hooper, R, Shannon, C.E, Tourtellotte, W.G, Singh, B.B, and Madesh, M (2020) Mitochondrial pyruvate and fatty acid flux modulate MICU1-dependent control of MCU activity. Science Signaling. Apr 21;13 (628). pii: eaaz6206. doi: 10.1126/scisignal.aaz6206.

    • Sun, Y.,  Nascimento Da Conceicao, V., Ahamad, N., Madesh, M and Singh B.B (2020) Spatial localization of Ca2+ channels and its modulators regulates neuronal physiology and contributes to its pathology. Current Opinion in Physiology. October Volume 17, Pages 50-62

    • Singh, B.B., Ohm, J., Quenum, F.Z., Chauhan, P., Kroese, F.G.M., Vissink, A., Ambrus, J.L, and Mishra, B (2020). Epigenetic-mediated loss of alpha-1 antiproteinase contributes to salivary gland dysfunction in primary Sjögren’s syndrome. Autoimmunity. May 25:1-13.

    • Sun, Y., Sukumaran, P., and Singh, B.B. (2020) Mg2+-induced cell survival is dependent on TRPM7 expression & function. Molecular Neurobiology. Jan;57(1):528-538.

    • Sun, Y., Kamat, A and Singh, B.B (2020) Isoproterenol-dependent activation of TRPM7 protects against neurotoxin-induced loss of dopaminergic cells. Frontiers in Physiology. 24 April 2020.

    • Conceicao, V N., Sun, Y.,  Zboril, EK Chapa, J D and Singh, B.B. (2020) Loss of Ca2+ entry via Orai-TRPC1 induces ER stress that initiate cytokine production and immune activation in macrophage cells Journal of Cell Science. 133 (5) jcs237610. doi:10.1242/jcs.237610ec.

    • Schaar, A., Sun, Y., Sukumaran, P., Rosenberger, T.D., Krout, D., Roemmich J.N., Brinbaumer, L., Claycombe-Larson, K and Singh, B.B. (2019) Ca2+ entry via TRPC1 is essential for cellular differentiation and modulates secretion via the SNARE complex. Journal of Cell Science. Jul 1;132 (13). pii: jcs231878. doi: 10.1242/jcs.231878.

    • Pu, Q., Zhao, Y., Sun, Y., Huang, T., Lin,P., Zhou, C., Qin, S., Wu, M and Singh, B.B (2019) TRPC1 Facilitates House Dust Mite-induced Airway Remodeling and Inflammation through Elevation of STAT3/NFkB Pathway. FASEB Journal. Jan;33 (1):1074-1085.

    • Sukumaran, P., Sun, Y., Zangbede, F.Q., Conceicao, V.N., Mishra, B., Singh, B.B. (2019) TRPC1 expression and function inhibits ER stress and cell death in salivary gland cells. FASEB BioAdvances Jan 1: 40–50.

    • Chauhan, A. , Sun, Y., Quenumzangbe, F., Sukumaran, P., Jondle, C.N., Chauhan, P, Birnbaumer, L, Sharma, J., Singh, B.B., and Mishra, B.B. (2018) M1-macrophage polarization is dependent on TRPC1-medited calcium entry. iScience (Cell Press) 8:85-102.

    • Sun, Y., Sukumaran, P., Selvaraj, S., Schaar, A., Cilz, N., Lei, S., and Singh, B.B (2018). TRPM2 inhibition protects neurotoxin MPP+-induced oxidative stress-mediated cell degeneration. Molecular Neurobiology. Jan; 55 (1):409-420.

    • Tripathi, J.K., Sharma, A., Sukumaran, P., Sun, Y., Mishra, B.B., Singh, B.B, and Sharma J (2018) Oxidant sensor cation channel TRPM2 regulates neutrophil extracellular trap formation and protects against pneumoseptic bacterial infection. FASEB Journal. Jun 15:fj201800605. doi: 10.1096/fj.201800605

    • Sun, Y., Selvaraj, S.,  Foster, J.,  Wu, M., Watt, J.A., Ohm, J and Singh, B.B (2018) BDNF induced differentiation of mesenchymal stem cells to dopaminergic neurons to exhibit increased sensitivity to MPP+ by inhibiting Ca2+ influx. Scientific Report Aug 6; 8 (1):11715.

    • Bhattacharya, A., Kumar, J., Qureshi, H., Sun, Y., Hermanson, K., Perley, D., Scheidegger, A., Singh, B.B and Dhasarathy, A (2018) The calcium channel ORAI3 mediates SNAIL expression during TGF-β induced EMT. Oncotarget. 9 (50): 29468-29483.

    • Sukumaran, P., Sun, Y., Antonson, N and Singh, B.B (2018) Dopaminergic neurotoxins induce cell death by attenuating NFκB-mediated regulation of TRPC1 expression and autophagy. FASEB Journal. 32 (3):1640-1652.

    • Sun, Y., Schaar, A., Sukumaran, P., Dhasarathy, A., and Singh, B.B (2018) TGFβ-induced epithelial-to-mesenchymal transition in prostate cancer cells is mediated via TRPM7 expression. Molecular Carcinogenesis 57(6):752-761.

    • Krout, D., Schaar, A., Sun, Y., Sukumaran, P., Roemmich, J.N., Claycombe-Larson, K.J and Singh, B.B (2017) Loss of TRPC1 is required for exercise-induced protection against high-fat diet-induced obesity and type II diabetes. Journal of Biological Chemistry. 292(50):20799-20807.

    • Bollimuntha S, Pani B, Singh BB (2017). Neurological and Motor Disorders: Neuronal Store-Operated Ca2+ Signaling: An Overview and Its Function. Advances in Experimental Medicine and Biology. 993: 535-556.

    • Sun, Y., Zhang, H., Selvaraj, S., Sukumaran, P., Lei, S., Birnbaumer, L., and Singh B.B. (2017). Inhibition of Cav1.3 channels by STIM1 in dopaminergic neurons is regulated by TRPC1. Journal of Neuroscience. 37(12):3364 –3377

    • Sukumaran, P., Sun, Y., Schaar, A., Selvaraj, S and Singh, B.B (2017). The role of TRPC channels in Parkinson’s disease In TRPC channels and Brain Diseases (Eds JunFeng Wang Springer Nature. Advances in Experimental Medicine and Biology, 976: 85-94.

    • Schaar, A., Sun, Y., Sukumaran, P., Dhasarathy, A., and Singh, B.B (2016) TRPC1 and STIM1 are critical for TGFb induced EMT migration through regulation of calpains. Oncotarget. Oct 25: 7 (49): 80554-80567.

    • Madeo, M., Sun, Y., Chandrasekar, I., Yarrow, A., Cordeiro, D., McCormick, E.M., Murarescu, C. C., Nolan, PM., Acevedo-Arozena, A., Singh, B.B., and Kruer, MC (2016). Loss-of-Function Mutations in FRRS1L Lead to an Epileptic-Dyskinetic Encephalopathy. American Journal of Human Genetics (Cell Press). Jun 2; 98 (6):1249-55.

    • Sukumaran, P., Schaar, A., Sun, Y., and Singh, B.B (2016). Functional role of TRP channels in modulating ER stress and Autophagy.  Cell Calcium. Aug; 60 (2):123-132.

    • Selvaraj, S., Sun, Y., Sukumaran, P., and Singh, B.B (2016). Resveratrol- activates autophagic cell death in cancer cells via down regulation of TRPC1-STIM1 and the mTOR pathway. Molecular Carcinogenesis. 55(5): 818-831

    • Sun, Y., Sukumaran, P., Schaar, A., and Singh, B.B (2015).TRPM7 and its role in neuro-degenerative diseases. Channels. 9:5, 244-252

    • Sukumaran, P., Sun, Y., Vyas, M., and Singh, B.B (2015). TRPC1-mediated Ca2+ entry is essential for the regulation of hypoxia and nutrient depletion-dependent autophagy in salivary gland cells. Cell Death and Disease. Mar 5;6: e1674. doi: 10.1038/cddis.2015.7.

    • Selvaraj, S., Singh, B.B., Bollensdorff, C., Suwaidi, J AL., Yacoub, M (2015). A critical role of TRPC1-ORAI1-STIM1 mediated store-operated calcium entry in cardiac hypertrophy. Journal of the American College of Cardiology. 65(10_S):. doi:10.1016/S0735-1097(15)60902-0

    • Zhou, X., Ye, Y., Sun, Y., Privratsky, B., Wang, W., Birnbaumer, L., Wu, M., and Singh, B.B (2015). TRPC1 deficiency impairs host defense and proinflammatory responses to bacterial infection by regulating PKC signaling Molecular and Cellular Biology. 35(16):2729-2739.

    • Chauhan, A., Quenum, F., Abbas, A., Bradley, DA., Nechaev, S., Singh, B.B., Sharma, J., Mishra, B.B (2015). Epigenetic modulation of microglial inflammatory gene loci in helminth-induced immune suppression. ASN Neuro. Jul 6;7(4). pii: 1759091415592126.

    • Sun, Y., Birnbaumer, L and Singh, B.B (2015). TRPC1 regulate calcium-activated chloride channels in salivary gland tissues and cells. Journal of Cellular Physiology. 230(11):2848-56.

    • Sun, Y., Chauhan, A., Sharma, J., Singh, B.B., and Mishra, BB (2014). Inhibition of store-operated Ca2+ entry in microglia by helminth antigens. Journal of Neuroinflammation. Dec 24;11:210.

    • Sun, Y., Sukumaran, P., Varma, A., Derry, S., Sahmoun, A. E., and Singh, B.B.  (2014). Cholesterol-induced activation of TRPM7 regulates cell viability, proliferation, and migration of human cancer cells Biochim Biophys Acta. 1843 (9), 1839–1850.

    • Chauhan, A., Sun, Y., Pani, B., Fredice, Q., Sharma, J., Singh, B.B., and Mishra, BB (2014). Helminth-induced suppression of macrophage activation is correlated with inhibition of calcium channel activity PLOS one. Jul 11;9 (7):e101023. doi: 10.1371

    • Sun, Y., Sukumaran, P., and Singh, B.B. (2014). Physiological function and characterization of TRPCs in non-excitable and excitable cells. Cells. 3(2), 455-475.

    • Pabelick, C. M., Singh, B.B, and Prakash. Y.S (2014). The role of Caveolae in the Airway in Calcium Signaling In Airway Smooth Muscle Cells (Editor Yong-Xiao) Springer Heidelberg 235-246.

    • Pani, B., Xibao L., Bollimuntha, S., Ingrid, R., Chen, O., Achen, V., Patel, HH, Ambudkar IS., and Singh, B.B. (2013). Impairment of TRPC1-STIM1 channel assembly and AQP5 translocation compromise agonist-stimulated fluid secretion in mice lacking caveolin1. Journal of Cell Science 126, 667-675.

    • Dasari, B Jaya Prasanthi R.P., Meiers, C., Singh, B.B. and Ghribi, O (2013). Differential effects of the estrogen receptor agonist estradiol on toxicity induced by enzymatically-derived or autoxidation-derived oxysterols in human ARPE-19 cells. Current Eye Research Online, 1–13.

    • Vohra, PK., Sathish, V., Thompson, MA. Kiel, A., Pabelick, CM, Singh, B.B and Prakash Y.S. (2013). TRPC3 Regulates Release of Brain-Derived Neurotrophic Factor from Human Airway Smooth Muscle Biochim Biophys Acta. Jul 27; 1833 (12): 2953-2960.

    • Sun, Y., Selvaraj, S., Varma, A., S Derry, S., Sahmoun, A. E., and Singh, B.B. (2013). The increase in serum Ca2+/Mg2+ ratio promotes cell proliferation of prostate cancer cells by activating the TRPM7 channel. Journal of Biological Chemistry 288, 255-263.

    • Mandavilli, S., Sahmoun, A. E., and Singh, B.B (2013). Association between serum calcium levels, calcium channels, microcalcification and breast cancer using breast imaging reporting and data system scores Breast Cancer treatment and therapy 5, 1 – 7.

    • Selvaraj, S., Sun, Y., Watt, J.A., Song, S., Lei, S., Birnbaumer, L and Singh, B.B (2012). Toxin- induced ER stress in mice involves downregulation of TRPC1 and inhibition of the AKT/mTOR signaling. Journal of Clinical Investigation. 122 (4) 1354-1367. Highlighted with a commentary in JCI and also recommended by the faculty of 1000.

    • Kost, GC., Selvaraj, S., Lee, YB., Kim, DJ.,  Ahn, C-H., and Singh, B.B. (2012). Clavulanic acid inhibits MPP+-induced ROS generation and subsequent loss of dopaminergic cells. Brain Research 1469:129-135

    • Singh, B.B, Christina M Pabelick and Y S Prakash. (2012). Canonical Transient Receptor Potential channel expression, regulation and function in vascular and airway diseases TRP channels in drug discovery (Editors Arpad Szallasi and Tamas Biro) Humana Press. Chapter 4, 61-87.

    • Sahmoun, A.B, and Singh, B.B. (2012). Calcium homeostasis and Cancer. Environment and Biotechnology (Editors Ram Prasad and Arvind Kumar) Lambert Academic Press. Chapter 4, 89-109.

    • Guo, Q, Shen, N, Yuan, K, Li, J, Wu, H, Zeng, Y, Fox, J,. Bansal, A. K, Gao, H, Singh, B B, and Wu, M (2012). Caveolin -1 plays a critical role in host immunity against Klebsiella pneumoniae by regulating STAT5 and Akt activity. Eur J Immunol 42 (6):1500-1511.
    • Bollimuntha, S, Pani, B., and Singh, B.B. (2012). The pathophysiological perspective of neuronal store operated Ca2+ signaling. Store-operated calcium entry pathways (Klaus Groschner and Christopher Romanian eds) Springer Heidelberg Chapter 26, 245-254.

    • Karlstad, J., Sun, Y and Singh, B.B. (2012). Ca2+ signaling: an outlook on the characterization of Ca2+ channels and their importance in cellular functions. Advances in Experimental Medicine and Biology. 740, 143-157.

    • Pani, B., Bollimuntha, S and Singh, B.B. (2012). The TR­(i)P to Ca2+ signaling just got STIMy: an update on STIM1 activated TRPC channels. Frontiers in Biosciences Jan 1; 17: 805-823.

    • Kost, G.C., Selvaraj, S., Lee, YB., Kim, DJ.,  Ahn, C-H., and Singh, B.B. (2011). Clavulanic acid enhances dopamine release in neuronal cells through a mechanism involving enhanced vesicle trafficking. Neuroscience letters 504, 170-175.

    • Dasari, B., Jaya Prasanthi R.P., Marwarha, G., Singh, B.B. and Ghribi, O (2011). Cholesterol-Enriched Diet Causes Age-Related Macular Degeneration-Like Pathology in Rabbit retina. BMC Ophthalmology. 11:22-29.

    • Yuan, K, Huang C, Fox J, Gaid M, Weaver A, Li G, Singh B.B, Gao H, Wu M (2011). The elevated inflammatory response is mediated by STAT3 and NF-κB in Caveolin-1 deficient mice in P. aeruginosa infection. Journal of Biological Chemistry 286 (24): 21814-25.

    • Bollimuntha, S., Selvaraj, S and Singh, B.B. (2011). Emerging role of canonical TRP channels in Neuronal function. Advances in Experimental Medicine and Biology. 704, 573-593.

    • Dasari, B Jaya Prasanthi R.P., Marwarha, G., Singh, B.B. and Ghribi, O (2010). The oxysterol 27-hydroxycholesterol increases beta-amyloid and oxidative stress in retinal pigment epithelial cells. BMC Ophthalmology. 10: 22-31.

    • Labyed Y, Kaabouch N, Schultz RR, Singh, B.B, and Milavetz B. (2010). An improved 1-D gel electrophoresis image analysis system. Advances in Experimental Medicine and Biology. 680:609-617.    

    • Sahmoun, A.B, and Singh, B.B. (2010). Does higher ratio of serum calcium to magnesium increases the risk of postmenopausal breast cancer? Medical Hypotheses 75, 315-318.

    • Liu, X., Ong, H.L., Pani, B., Katherine Johnson, Abinav Reddy., William B Swaim., Singh, B.B., and Ambudkar I.S. (2010). Effect of cell swelling on ER/PM junctional interactions and channel assembly involved in SOCE. Cell Calcium. 47, 491-499.

    • Selvaraj, S., Sun, Y., and Singh, B.B. (2010). TRPC channels and their implication in neurological diseases. CNS and Neurological Disorders-Drug targets. Volume 9, 94-104.

    • Pani, B., Brazer, So-Ching., Xibao Liu, Ong, H.L., Ambudkar IS., and Singh, B.B (2009). Activation of TRPC1 by STIM1 in ER-PM micro domains involves the release of the channel from its scaffold Caveolin1. Proceedings National Academy of Science. 106, 20087-20092.  

    • Selvaraj, S., Watt, J, and Singh, B.B (2009). TRPC1 inhibits apoptotic cell degeneration induced by dopaminergic neurotoxin MPTP/MPP+ Cell Calcium. 46, 209-218.

    • Ambudkar, I.S, Ong, H.L., and Singh, B.B. (2009). Molecular and functional determinants of Ca2+ signaling microdomains Signal Transduction: Pathways, Mechanisms, and Diseases, (ed Sitaramayya A). Springer, Heidelberg Chapter 13, 237-253.
    • Pani, B, and Singh, B.B. (2009). Lipid rafts/caveolae as microdomains of calcium signaling. Cell Calcium. 45, 625-633. The second most cited article till date.

    • Pani, B, and Singh, B.B. (2009). Principles and applications of Proteomics: Molecular determination of TRPC1 target proteins. Textbook on Molecular Biotechnology (Ashok Chauhan and Ajit Varma eds) IK International Publishing house. Chapter 53, 1125-1138.

    • Bandyopadhyay B.C., Ong, H.L., Lockwich, T.P., Liu, X., Paria, B., Singh, B.B., and Ambudkar, I.S., (2008). TRPC3 controls agonist-stimulated intracellular Ca2+ release by mediating the interaction between IP3R and RACK1. Journal of Biological Chemistry. 283(47):32821-32830.

    • Pani B., Ong, H.L., Liu, X., Rauser, C., Ambudkar, I.S. and Singh, B.B (2008). Lipid Rafts Determine Clustering of STIM1 in ER-Plasma Membrane Junctions and regulation of SOCE. Journal of Biological Chemistry. 283; 17333-17340.

    • Pani, B, and Singh, B.B. (2008). Dariers disease: a calcium-signaling perspective. Cellular and Molecular Life Sciences. 65; 205-211.

    • Liu, X., Cheng, O., Bandyopadhyay B.C, Pani B., Dietrich, A., Paria, B., Swaim, W., Birnbaumer, L., Singh, B.B., and Ambudkar, I.S. (2007). An essential role for TRPC1 in store-operated Ca2+ entry and regulation of salivary gland fluid secretion. Proceedings National Academy of Science, USA. 104; 17542-17547.

    • Ong, H.L., Liu, X, Atanasova, K-T., Singh, B.B., Bandyopadhyay, B., Swaim, W.D., Hegde, R.S., Sherman, A, and Ambudkar, I.S. (2007). Relocalization of STIM1 for activation of store-operated Ca2+ entry is determined by the depletion of subplasma membrane-endoplasmic reticulum Ca2+ store. Journal of Biological Chemistry. 282; 12176-12185.

    • Labyed, Y., Kaabouch, N., Schultz, R.R., and Singh, B.B. (2007). “Automatic segmentation and band detection of protein images based on the standard deviation profile and its derivative,” IEEE Electro/Information Technology Proceedings, article. no. 4374497, pp. 577-582.

    • Ong, H.L., Cheng, K.T., Liu, X., Bandyopadhyay, B., Paria, B.C., Soboloff, J., Pani B., Gwasck, Y., Srikanth, S., Gill, D., Singh, B.B., and Ambudkar, I.S (2007). Dynamic assembly of the TRPC1/STIM1/Orai1 ternary complex is involved in store-operated Ca2+ influx. Journal of Biological Chemistry. 282; 9105-9116.

    • Jara, J.H, Singh, B.B., Floden, A.M, Hansen, J, and Combs, C.K. (2007). Tumor necrosis factor alpha stimulates NMDA receptor activity in mouse cortical neurons resulting in ERK-dependent death. Journal of Neurochemistry. 100; 1407-1420.

    • Pani B., Cornatzer E., Cornatzer W., Shin DM., Pittelkow MR., Ambudkar IS., and Singh B.B. (2006). Upregulation of TRPC1 following SERCA2 gene silencing promotes cell survival. Molecular Biology of the Cell. 17; 4446-4458.

    • Bollimuntha, S., Ebadi, M., and Singh, B.B (2006). TRPC1 protects SH-SY5Y cells against salsolinol induced cytotoxicity by inhibiting apoptosis. Brain Research. 1099; 141-149.

    • Liu, X., Bandyopadhyay, B., Nakamoto, T., Singh, B.B.,  Liedtke, W., Melvin, J.E., and Ambudkar, I.S. (2006). A role of AQP5 in activation of TRPV4 by hypotonicity: concerted involvement of AQP5 and TRPV4 in the regulation of cell volume recovery. Journal of Biological Chemistry. 281; 15485-15495.

    • Liu, X., Bandyopadhyay, B., Singh, B.B., Groschner, K., and Ambudkar, I.S. (2005). Molecular analysis of a store-operated and OAG sensitive non-selective cation channel. Journal of Biological Chemistry. 280; 21600-21606.

    • Bollimuntha, S., Cornatzer, E, and Singh, B.B (2005). Plasma membrane localization and function of TRPC1 depend on its interaction with tubulin in epithelium cells. Visual Neuroscience. 22; 163-170.

    • Bollimuntha, S., Shavali, S., Sharma, S.K., Ebadi. M., and Singh, B.B (2005). TRPC1-mediated inhibition of MPP+ neurotoxicity in human SH-SY5Y neuroblastoma cells.  Journal of Biological Chemistry. 280; 2132-2140.

    • Singh, B.B et al., (2004). Moving TRPs to the membrane, Science. 305; 1680.

    • Ebadi, M., Brown-Borg, H., Refaey, H.E., Singh, B.B., Garrett, S., Shavali, S and Sharma, SK (2005). Metallothionein-mediated neuroprotection in genetically engineered mice models of Parkinson’s disease and aging. Molecular Brain Research. 134; 67-75.

    • Singh, B.B., Lockwich, T., Bandyopadhyay, B., Liu, X., Bollimuntha, S., Brazer, SC., Combs, C., Sheng, Z., Knepper, M., Ambudkar, S, and Ambudkar, I.S. (2004).  VAMP-2-dependent exocytosis is involved in plasma membrane insertion of TRPC3 channels and contributes to the agonist-stimulated Ca2+ influx. Molecular Cell. 15; 635-646. A summary of this article was published in Nature Cell Biology and Science SKTE.

    • Itagaki, K., Kannan, K.B., Singh B.B and Hauser C.J. (2004). Cytoskeletal reorganization internalizes multiple Transient Receptor Potential channels and blocks calcium entry into human neutrophils. Journal of Immunology. 172; 601-607.

    • Ambudkar IS, Brazer SC, Liu X, Lockwich T, and Singh, B. B. (2004). Plasma membrane localization of TRPC channels: the role of caveolar lipid rafts. Mammalian TRP Channels as Molecular Targets (Chadwick DJ and Goode J, eds) Wiley InterScience Publications. 258; 63-70.

    • Brazer, S.W., Singh B.B., Liu, X., Swaim, W & Ambudkar I.S (2003). Caveolin-1 contributes to assembly of store-operated Ca2+ influx channels in regulating plasma membrane localization of TRPC1. Journal of Biological Chemistry. 278; 27208-15.

    • Singh, B.B*., Liu, X* & Ambudkar, I.S. (2003). Acidic amino acid residues in the S5-S6 region of TRPC1 contributes to the store-operated Ca2+ influx. Journal of Biological Chemistry. 278; 11337-11343. *both contributed equally

    • Singh, B.B., Liu, X., Tang, J., Zhu, M.X & Ambudkar, I.S. (2002). .Calmodulin regulates Ca2+-dependent feedback inhibition of store-operated Ca2+ influx by interaction with a site in the C-terminus of TRPC1. Molecular Cell.  9; 739-750.

    • Singh, B.B., Zheng, C., Liu, X., Lockwich, T., Liao, D., Zhu, M., Birnbaumer, L & Ambudkar, I.S. (2001). TRPC1 dependent enhancement of salivary gland fluid secretion: Role of store-operated calcium entry. FASEB Journal. 15; 1652-1654.

    • Singh, B.B*., Lockwich, T*., Liu, X & Ambudkar, I.S. (2001). Stabilization of cortical actin induces internalization of TRPC3-associated caveolar Ca2+ signaling complex and loss of Ca2+ influx without disruption of TRPC3-IP3R association. Journal of Biological Chemistry. 276; 42401-42408. *both contributed equally

    • Yunfei, C., Singh, B.B., Aslanukov, A., Zhao, H and Ferreira, P.A. (2001). The docking of heterotetrameric kinesins to RanBP2 is mediated via a novel RanBP2 domain. Journal of Biological Chemistry. 276; 41594-41602. 

    • Singh, B.B; Curdt, I; Shomburg, D; Bisen, P.S and Böhme, H (2001). Valine 77 of heterocystous ferredoxin FdxH2 in Anabaena variabilis ATCC 29413 is critical for its oxygen sensitivity. Molecular and Cellular Biochemistry. 217; 137-142.

    • Singh, B.B., Liu, X., & Ambudkar, I.S (2000). Expression of truncated TRPC1: Evidence that the TRPC1 C-terminus modulates store-operated Ca2+ entry. Journal of Biological Chemistry. 275; 36483 - 36486.

    • Lockwich, T*, Liu, X*., Singh, B.B., Jadlowiec, J. Weiland, S., & Ambudkar I.S (2000). Assembly of TRPC1 in a Signaling Complex Associated with Caveolin-Scaffolding Lipid Raft Domains. Journal of Biological Chemistry. 275; 11934-11942. *both contributed equally

    • Liu, X*., Wang, W*., Singh, B.B., Lockwich, T., Jadlowiec J., Connell, B.O., Wellner, R., Zhu, X., & Ambudkar, I.S (2000). TRPC1 a candidate protein for the store-operated Ca2+ influx Mechanism in salivary gland cells. Journal of Biological Chemistry. 275; 3403-3411. *both contributed equally

    • Curdt, I., Singh, B.B., Jakoby, M., Hachtel, W & Böhme, H (2000). Identification of amino acid residues of nitrite reductase from Anabaena sp. PCC 7120 involved in ferredoxin binding.  Biochimica et Biophysica Acta (BBA) - Bioenergetics. 1543; 60-68.

    • Varma A., Singh B.B., Karnani N., Lichtenberg, H., Hoefer M., Magee B.B. & Prasad R. (2000). Molecular cloning and functional characterization of a glucose transporter, CAHGTI, of Candida albicans. FEMS Microbiology letters.182; 5-21

    • Liu, X., Singh, B.B & Ambudkar, I.S (1999). ATP-dependent activation of KCa and ROMK-type KATP channels in human submandibular gland ductal cells. Journal of Biological Chemistry. 274; 25121-25129.

    • Singh, B.B., Patel, H.H., Ropeman, R., Schick, D. & Ferreira, P.A. (1999). The Zinc finger cluster domain of RanBP2 is a specific docking site for Nuclear Export Factor, Exportin-1. Journal of Biological Chemistry. 274; 37370-37378.

    • Singh, B.B., Curdt, I., Jakobs, C., Bisen, P.S. & Böhme, H (1999). Identification of amino acids responsible for oxygen sensitivity of ferredoxins from Anabaena variabilis using site-directed mutagenesis Biochimica et Biophysica Acta (BBA) - Bioenergetics.1412; 288-294.

    • Sengupta, L.K., Singh, B.B., Mishra, R., Pandey, P.K., Singh, S., Sengupta, S. & Bisen, P.S (1998). Calcium- dependent metabolic regulations in prokaryotes indicate conserved nature of calmodulin gene. Indian Journal of Experimental Biology. 36; 136-147.

    • Singh, B.B, S. Singh and P.S. Bisen (1998). why does diazotrophic cyanobacterium in its immobilized state liberate ammonium? (Ed.) V.K. Jain Wisdom Publishing House. New Delhi 352-358.

    • Bisen, P.S; Singh, B.B; Sengupta, L.K; Mishra, R; Sengupta, S; Pandey, P.K & Singh, S (1997). Calcium- calmodulin: An overview with reference to microbes In: Microbiological Prospects and Applications (edited by A. Varma) IBH & Oxford Publications. New Delhi. pp 125-137.

    • Singh, B.B., Pandey, P.K., Singh, S. & Bisen, P.S (1997). Calcium-induced physiological and biochemical changes in the cyanobacterium Nostoc MAC. Indian Journal of Experimental Biology. 35; 881-885.

    • Singh, B.B., Pandey, P.K., Singh, S. & Bisen, P.S (1996). Evidence for the nitrate assimilation dependent nitrite excretion in cyanobacterium Nostoc MAC. World Journal of Microbiology and Biotechnology. 12; 285-287.

    • Singh, B.B., Pandey, P.K., Singh, S, & Bisen, P.S (1996). Regulation of nitrate uptake and nitrite efflux in the cyanobacterium Nostoc MAC. Journal of Basic Microbiology. 36; 433-438

    • Singh, B.B., Pandey, P.K., Singh, S. & Bisen, P.S. (1996). Calcium/calmodulin antagonist-induced changes in the cyanobacterium Nostoc MAC. Physiology & Molecular Biology of Plants. 2; 75 - 78.

    • Pandey, P.K., Singh, B.B., Mishra, R. & Bisen, P.S. (1996). Ca2+ uptake and its regulation in the cyanobacterium Nostoc MAC. Current Microbiology. 32; 332-336.

    • Singh, S., Singh, B.B.  & Bisen, P.S. (1995). Copper-induced changes in the urea uptake and urease activity in the cyanobacteria Anabaena doliolum and Anacystis nidulans:  Interaction with sulfur containing amino acids. Biomedical and Environmental Science. 8: 158 - 163.

    • Pandey, P.K., Singh, B.B., Singh, S.  & Bisen, P.S. (1995). NO2- efflux and its regulation in the cyanobacterium Nostoc MAC Current Microbiology. 31; 119 -123.

    • Singh, S., Singh, B.B., & Bisen, P.S. (1995). The role of ammonium assimilation in the urea inhibition of nitrogenase activity in cultured cyanobiont Nostoc ANTH. Indian Journal of Experimental Biology. 33; 33 -39.

    • Singh, S., Singh, B.B., & Bisen, P.S. (1994). The role of membrane potential in ammonium inhibition of nitrogenase activity in the cultured cyanobiont Nostoc ANTH.  World Journal of Microbiology & Biotechnology (Rapid communication of Oxford Ltd. U.K).  10; 600.